Determination of morphine in serum and cerebrospinal fluid by gas chromatography and selected ion monitoring after reversed-phase column extraction
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منابع مشابه
Determination of DiazaCon in quail feed and quail serum by ion pair reversed-phase chromatography.
Liquid chromatographic (LC) methods were developed for quantitating the potential avian contraceptive DiazaCon in quail feed and serum. DiazaCon was extracted from ground quail feed with basic n-butyl chloride. The n-butyl chloride extract was evaporated to dryness. The DiazaCon residues were dissolved in an aqueous methanolic ion pairing solution and quantitated by LC at 206 nm. Avian sera was...
متن کاملDetermination of corticosterone in rat and mouse plasma by gas chromatography-selected ion monitoring mass spectrometry.
A simple, highly sensitive and specific method based on gas-chromatography-selected ion monitoring (SIM) mass spectrometry has been developed for the quantitation of corticosterone in rat and mouse plasma. After extraction of the plasma with ethyl acetate, the residue was trimethy-silylated with pentafluorobenzyl hydroxylamine-trimethylsilyl (PFBO-TMS). Detection of the derivatives was accompli...
متن کاملDetermination of testosterone in human hair by gas chromatography-selected ion monitoring mass spectrometry.
A method for the determination of testosterone in human hair by gas chromatography-mass spectrometry using d3-testosterone as internal standard is described. Our method consisted of alkaline digestion, fast liquid-liquid extraction, LH-20 chromatography and derivatization with heptafluorobutyric anhydride. Quantification was achieved by selected ion monitoring of m/z 680 (testosterone) and m/z ...
متن کاملMicrodetermination of cholesterol and phospholipid in cerebrospinal fluid and serum by silicic acid column chromatography.
A method is presented for the determination of cholesterol and phospholipid, which requires 5 I . of human serum or 1-2 ml. of cerebrospinal fluids. With this method 5-100 zg. of cholesterol and phospholipid can be separated by a modified silicic acid column after elution of the mixture with 1 ml. of chloroform and 3 ml. of methanol. Recovery for 24.6 sg. of cholesterol and 30.5 g. of phospholi...
متن کاملDetermination of delta-aminobutyric acid and other amino acids in cerebrospinal fluid of pediatric patients by reversed-phase liquid chromatography.
The reversed-phase liquid-chromatographic system described here is capable of resolving the neurotransmitter amino acids aspartic acid, glutamic acid, and gamma-aminobutyric acid (GABA) plus 21 other amino acids in cerebrospinal fluid (CSF) in a single analysis. The amino acids, derivatized with o-phthalaldehyde, are separated in 65 min. Concentrations of glutamine less than or equal to 600 mum...
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ژورنال
عنوان ژورنال: Journal of Chromatography B: Biomedical Sciences and Applications
سال: 1984
ISSN: 0378-4347
DOI: 10.1016/0378-4347(84)80081-x